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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Bone. 2018 Oct 17;120:125–136. doi: 10.1016/j.bone.2018.10.013

Fig. 6: TNAP Deficiency Promotes FGFR2 Expression via FGF Signaling.

Fig. 6:

(A) Immunoblotting shows diminished FGFR2 expression in MC3T3E1 cells expressing TNAP shRNA and in calvarial progenitor cells isolated from TNAP−/− mice, as compared to control cells. (B) Transduction of MC3T3E1 cells with a TNAP expression adenovirus diminishes FGFR2 expression in both TNAP shRNA and nontarget shRNA expressing cells. (C) Treatment with the actinomycin D inhibitor of transcription decreases FGFR2 protein expression in both TNAP deficient and control cells. (D) Treatment with the actinomycin D inhibitor of transcription decreases FGFR2 mRNA expression in both TNAP deficient and control cells. (E) FGF2 but not Pi treatment decreases FGFR2 expression in both TNAP deficient and control cells. Quantified cell data are represented as mean ± SD.