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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Exp Eye Res. 2018 Oct 15;179:18–24. doi: 10.1016/j.exer.2018.10.009

Figure 3: Phagocytic function in differentiated ARPE-19 cells.

Figure 3:

(A) Micrographs of opsin-labeled ARPE-19 cells (before and after cell permeabilization) cultured on Transwell inserts in MEM or MEM-Nic, following exposure to porcine POSs for 2 h and fixed immediately (pulse), or fixed after a further 1-h chase period in the absence of POSs. Surface-bound POSs appear in yellow whereas internalized POSs appear in red. (B) Bar graph showing quantification of POSs. Data were normalized relative to the total number of POSs (bound and ingested) after the pulse (n = 7 fields of view, aggregated from separate experiments). The relative number of ingested POSs (i.e. POS phagosomes) after the pulse (n = 4) and the total number of POSs after the chase (n = 7) are also shown. Scale bar, 10 μm. Error bars in (B) represent ± S.E.M. Each field of view (n) contained ≥ 100 cells.