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. Author manuscript; available in PMC: 2019 Nov 28.
Published in final edited form as: Chem Rev. 2018 Oct 29;118(22):10840ā€“11022. doi: 10.1021/acs.chemrev.8b00074

Figure 147.

Figure 147.

(Left) Crystal structure of the oxy-F33Y-CuBMb (1.27 ƅ resolution; PDB ID: 5HAV) with two water molecules in the active site pocket. (Center) Cryoreduction method for formation and trapping of (hydro)peroxo intermediates (X = active site His or Tyr). (Right) The EPR spectra of oxy-F33Y-CuBMb cryoreduced at 77 K showing a ferric-hydroperoxo (H-bonded to an active site water, green) which decays (and loses H-bond stabilization) upon stepwise annealing. Adapted from ref 1122. Copyright 2016 American Chemical Society.