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. 2018 Dec 4;10(2):163–169. doi: 10.1111/1759-7714.12925

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© 2018 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Typical circulating tumor cells (CTCs) in the pulmonary vein (PV) were visualized using green fluorescent protein (GFP) expression. Blood specimens were incubated with oHSV1‐hTERT‐GFP and then stained with allophycocyanin‐labeled anti‐epithelial cell adhesion molecule (EpCAM) antibody. From left to right, pictures were taken sequentially in white light, then using green (GFP) and red (EpCAM) fluorescence. The picture on the far right depicts the overlap of all fluorescence. Scale 10 μm.