Figure 5.

miR-1307-3p plays its oncogenic role through SMYD4. (A) SMYD4 protein level was dramatically increased by transfection of SMYD4 expression plasmid. Stable expressing miR-1307-3p MCF10A cells were transfected with MYD4 expression plasmid or vector. After 72 hours of transfection subjected to Western blot. (B) miR-1307-3p induced stimulation of cell proliferation was abrogated by SMYD4 overexpression in MCF10A cells. Stably expressing miR-1307-3p MCF10A cells were transfected with SMYD4 expression plasmid (MCF10A/miR-1307-3p/SMYD4) or empty vector (MCF10A/miR-1307-3p), then subjected to cell proliferation assay. ***, p<0.001 compared to MCF10A/miR-1307-3p group. (C) miR-1307-3p induced foci forming in soft agar was blocked by SMYD4 overexpression in MCF10A cells. Stably expressing miR-1307-3p MCF10A cells were transfected with SMYD4 expression plasmid (miR-1307-3p/SMYD4) or empty vector (miR-1307-3p), then subjected to cell proliferation assay. Ctrl, MCF10A cells that infected with lentivirus empty vector. (D) miR-1307-3p stimulated tumor formation of MCF10A cell in nude mice was blocked by SMYD4 overexpression in MCF10A cells. 5×10⁵ indicated cells were subcutaneously injected to per mouse and this experiment continued 6 months.