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. 2019 Jan 1;10(2):355–366. doi: 10.7150/jca.27832

Figure 6.

Figure 6

Upregulation of hepatic CDCA5 expression in HCC cell lines and patients with HCC and knockdown of CDCA5 in Huh7 and Hep3B. (A) Expression of CDCA5 mRNA in 4 human HCC cell lines and 2 normal human hepatic cells was evaluated by qRT-PCR. (B) Western blots show the CDCA5 protein level compared to Tubulin in HCC cell lines and normal hepatic cells. (C) Representative images of immunohistochemistry staining of liver sections of HCC tissues (n=81) and paired adjacent normal tissues (n=81). Original magnification, ×200. Upper panel, HCC tissues; Lower panel, adjacent normal tissues. (D and E) Western blots and qRT-PCR showing the effect of RNAi on CDCA5 expression. There different siCDCA5s at 50 nM were transfected into human HCC cell lines Huh7 and Hep3B using Lipofectamine 3000. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.