Immunohistochemical localization of CYP46A1 in the retina. A: Representative stains of retinal flat mounts (top row) and cross sections (bottom row) for CYP46A1 (green) and CD31 (red). B: Representative stains of retinal cross sections for CYP46A1 (green) and neuronal-specific nuclear protein (NeuN; red). The Cyp46a1−/− retina was used as a negative control. Nuclei were stained with DAPI (blue). n = 3 (A and B). Scale bars: 10 μm (A, flat mounts); 20 μm (A and B, cross sections). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer.