Figure 4.

Vascular abnormalities and vascular leakage in the Cyp46a1^−/− retina, as assessed by fluorescein angiography and immunohistochemistry. A: Representative images of blood vessel tortuosity (white arrow), microaneurysm-like fluorescent spots (pink arrows), and area of vascular leakage (yellow ovals). B: Representative images of increased vascular permeability, as assessed by stains for albumin (green) and isolectin B4 (red). Nuclei were stained with DAPI (blue). n = 3 (A and B). Scale bars = 50 μm (B). 2°, Control stain using secondary antibody only; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, photoreceptor inner segment; NI, control stain using nonimmune serum; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, photoreceptor outer segment; RPE, retinal pigment epithelium.