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. 2019 Feb;189(2):405–425. doi: 10.1016/j.ajpath.2018.10.013

Figure 8.

Figure 8

Macrophage/microglia activation in the Cyp46a1−/− retina. A and B: Representative stains of retinal flat mounts for F4/80 (red; A) and retinal cross sections for Iba1 (red; B); nuclei were stained with Hoechst (blue) and DAPI (blue), respectively. C: Enlargement of the boxed area in B showing wild-type retina with Iba1-positive cells. n = 3 (A and B). Scale bars = 50 μm (B). Original magnification, ×200 (A). 2°, Control stain using secondary antibody only; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; NI, control stain using nonimmune serum; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium.