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. 2019 Feb;189(2):405–425. doi: 10.1016/j.ajpath.2018.10.013

Table 1.

Primers for Quantitative Real-Time PCR

Gene Forward primer Reverse primer Species
Abca1 5′-AGGCCGCACCATTATTTTGTC-3′ 5′-GGCAATTCTGTCCCCAAGGAT-3′ M
Abcg1 5′-ATTTCATCGTCCTGGGCATCT-3′ 5′-CGGATTTTGTATCTGAGGACGAA-3′ M
Actβ 5′-TGTTACCAACTGGGACGACATG-3′ 5′-TTGTAGAAGGTGTGGTGCCAGA-3′ M
Aim 5′-CGTTAGAAGAAGAAGGTCGTTGGA-3′ 5′-AGATATGCAAGCGACCCATCTAC-3′ M
Apod 5′-TGAAGCCAAACAGAGCAACGT-3′ 5′-GGCATCAACGGGAAGAACTG-3′ M
Apoe 5′-GGCCCAGGAGAATCAATGAG-3′ 5′-CCTGGCTGGATATGGATGTTG-3′ M
ArgII 5′-GACCACAGCCTGGCAATAGGT-3′ 5′-TCAACCCAGATGACACAGAGATCT-3′ M
Ccl2 5′-TCACTGAAGCCAGCTCTCTCTTC-3′ 5′-GTGAACAGCAGGCCCAGAA-3′ M
Cox-2 5′-CACCTCAAGAACATCCAGAGCTT-3′ 5′-CTCGCGACCATTCTTGAGTGT-3′ M
Cxcl1 5′-CACCTCAAGAACATCCAGAGCTT-3′ 5′-CTCGCGACCATTCTTGAGTGT-3′ M
CYPA 5′- GGTCCCAAAGACAGCAGAAA-3′ 5′-GTCACCACCGTCACACATAAA-3′ H
CypA 5′-GAGCACTGGAGAGAAAGGATTT-3′ 5′-GACTTGCCACCAGTACCATTAT-3′ B
CYP27A1 5′-CACAAACTCCCGGATCATAGAA-3′ 5′-CACATAGTGGAACACAAAC-3′ H
Cyp27a1 5′-AGTAGACACGACATCCAACAC-3′ 5′-CACACCCACCACTTCCTTAT-3′ B
CYP46A1 5′-GAGTCCTGAGTCGCTTAAGAAG-3′ 5′-CGAAGAGTCTCTCACCAAACA-3′ H
Cyp46a1 5′-CAAGCCCAAGTTCACCTACT-3′ 5′-TCACCTCCATCTGAGCAAAC-3′ B
Glut4 5′-ACCGGATTCCATCCCACAA-3′ 5′-CATGCCACCCACAGAGAAGA-3′ M
Idol 5′-GGAGCATGTCCAGCACGTCTA-3′ 5′-GTGCAGGACGCATCAGATGA-3′ M
Il-1β 5′-GGTCAAAGGTTTGGAAGCAG-3′ 5′-TGTGAAATGCCACCTTTTGA-3′ M
Il-6 5′-AGTTGCCTTCTTGGGACTGA-3′ 5′-TCCACGATTTCCCAGAGAAC-3′ M
iNOS 5′-GCCACCAACAATGGCAACA-3′ 5′-CGTACCGGATGAGCTGTGAA-3′ M
Lxrα 5′-AGCGTCCATTCAGAGCAAGTG-3′ 5′-CACTCGTGGACATCCCAGATCT-3′ M
Lxrβ 5′-ACTCGGAGCAGGTCTTTGCAT-3′ 5′-CCTACTCGTGCACATCCCAGAT-3′ M
Mertk 5′-AAAGGTCCCCGTCTGTCCTAA-3′ 5′-TGGACACCGTCAGTCCTTTGT-3′ M
Srebp1a 5′-ATGGACGAGCCACCCTTCA-3′ 5′-AAGTCACTGTCTTGGTTGTTGATGA-3′ M
Srebp1c 5′-ACGGAGCCATGGATTGCA-3′ 5′-AAGTCACTGTCTTGGTTGTTGATGA-3′ M
Tnfα 5′-GGTCTGGGCCATAGAACTGA-3′ 5′-CAGCCTCTTCTCATTCCTGC-3′ M

B, bulls; H, humans; M, mice.