Supplemental Figure S1.

Antibiotic (ABX) disruption of gut microbiota does not affect cortical bone. Twelve-week–old male vehicle (VEH)– and ABX-treated mice and female VEH- and ABX-treated mice were euthanized; specimens were harvested for analysis. A–C: Micro–computed tomographic (μCT) analyses of the tibia middiaphysis cortical bone in male VEH- and ABX-treated mice. A: Representative reconstructed cross-sectional images in male VEH- and ABX-treated mice. B: Male cortical area fraction (Ct.Ar/Tt.Ar). C: Male cortical thickness (Ct.Th). D–F: μCT analyses of the tibia middiaphysis cortical bone in female VEH- and ABX-treated mice. D: Representative reconstructed cross-sectional images in female VEH- and ABX-treated mice. E: Female Ct.Ar/Tt.Ar. F: Female Ct.Th. Unpaired t-test was used. Data are expressed as means ± SEM (B, C, E, and F). n = 4 per group (A–F).