Figure 6.

Antibiotic (ABX) alteration of gut microbiota impact of innate and adaptive immune cell profile. Twelve-week–old male vehicle (VEH)– and ABX-treated mice were euthanized; specimens were harvested for analysis. A–H: Flow cytometric analysis of lymphocyte immune cell populations in bone marrow, spleen, and mesenteric lymph nodes (mLNs). Percentages of CD3⁺CD8⁻CD4⁺ (helper) T cells (A); CD3⁺CD4⁻CD8⁺ (cytotoxic) T cells (B); CD3⁻NK1.1⁺CD11b⁺ NK cells (C); CD3⁺CD4⁺CD25⁺FoxP3⁺ T_REG cells (D); CD3⁺CD4⁺CD183⁺T-bet⁺ Th1 cells (E); CD3⁺CD4⁺RORγT⁺AHR⁻ Th17 cells (F); B220⁺IgD⁺IgM⁺CD40⁺ (activated) B cells (G); and B220⁺IgD⁻IgM⁺CD40⁺ (memory) B cells (H). Percentages are expressed relative to total live gated lymphocytes. I–L: Flow cytometric analysis of innate immune cell populations in bone marrow, spleen, and mLNs. Percentages of CD11b⁺Ly6G⁻F4/80⁺Ly6C⁺ (proinflammatory) monocytes (I); CD11b⁺ MHC-class II⁺CD64⁺ M1 macrophages (J); CD11b⁺MHC II⁺CD64⁻CD206⁺ M2 macrophages (K); and CD11c⁺MHC-class II⁺CD317⁺ (activated plasmacytoid DCs; L). Cell percentages are expressed relative to total live-gated monocyte cells. Unpaired t-test was used. Data are expressed as means ± SEM (A–L). n = 4 to 5 per group (A–L). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 versus vehicle.