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. 2019 Jan 10;13(2):338–357. doi: 10.1002/1878-0261.12406

Figure 2.

Figure 2

Validation of hnRNP‐K O‐GlcNAcylation. CCA cells (KKU‐213 and KKU‐214) were treated with PUGNAc or vehicle for 24 h. The cell lysates were immunoprecipitated with either (A) anti‐OGP or (B) anti‐hnRNP‐K and probed with anti‐hnRNP‐K and anti‐OGP. Human immunoglobulin G (IgG) isotype was used as the controls of the specificity of the antibodies that were used in the immunoprecipitation assay. (C) The sWGA pull‐down assay was performed using sWGA‐conjugated agarose and probed with anti‐hnRNP‐K, sWGA, and anti‐OGP. GlcNAc neutralization was used to examine the specific binding of sWGA to the OGPs.