Figure 3.

The comparison of responses to chemotherapy alone and combination with targeted therapy in PH048 tumor harboring amplification of NRG3 gene. (A) Tumor volume changes in response to chemotherapy compared with those in untreated mice. (B) Treatment responses to combination of chemotherapy and anti‐HER targeted therapy versus chemotherapy alone. The time of administration for each treatment and the duration are shown by arrows. Volumes are expressed as percentage of initial volume at the start of the treatment, as mean ± SD. * P = 0.02 and ** P = 0.04. Student's t‐test was used to compare the differences. (C) Immunostaining showing levels of HER2 in PH048 mice treated with targeted + chemo combination (left) and with chemotherapy only (right). Scale bars: 100 μm. (D) Changes in the levels of HER2 and pHER2 elicited by each treatment as determined by immunoblotting analysis. The protein levels are normalized to GAPDH level.