Figure 3.

Role of TAMs and tumor cells in extracellular LPA production. (A, B) Levels of 16:0, 18:1, and 20:4 LPAs in culture supernatants from tumor cells or TAMs. Cells cultured in OCMI medium supplemented with 50% ascites for 24 h were incubated in ascite‐free medium containing 0.1% fatty acid‐free bovine serum albumin for an additional 24 h prior to adding either solvent or LPCs (16:0 and 18:1 mixture), and culture supernatants were analyzed by LC‐MS/MS 8 h (A) and 24 h (B) later. Horizontal bars represent the mean of two biological duplicates and vertical lines the range. (C) Heatmap depicting the correlation of the abundance of CD163+CD206+ (left) and CD163‐CD206‐ TAMs (right) in ascites with the ascites levels of the indicated LPA‐generating enzymes (Spearman correlation, ρ). IL‐6 and IL‐10 were included as known mediators prognostic of a poor survival in HGSC, and CXCL9 and IFNγ as cytokines prognostic of a favorable clinical outcome (Lieber et al., 2018; Reinartz et al., 2016; Worzfeld et al., 2017).