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. 2018 Nov 15;13(2):246–263. doi: 10.1002/1878-0261.12400

Figure 3.

Figure 3

Cytotoxicity in vitro of LR004‐VC‐MMAE. (A) The cell viability analysis of LR004 (blue line), LR004‐VC‐MMAE (red line), and rituximab‐VC‐MMAE (purple line) to KYSE520 and KYSE150 cells. The cell viability analysis of LR004‐VC‐MMAE to A431 and Karpas 299 cells. Karpas 299 cells were used as the negative control of cell line which expressed CD30 antigen on the cell surface. The cell viability was assessed using the CCK‐8 assay for 48 h. (B) The induction of apoptosis analysis in the KYSE520 and KYSE150 cells was detected by flow cytometry. The cells were treated with various concentrations of LR004‐VC‐MMAE for 24 h. (C) The cell cycle arrest analysis in the KYSE520 and KYSE150 cells was detected by flow cytometry. The cells were treated with various concentrations of LR004‐VC‐MMAE for 24 h.