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. 2019 Jan 1;10(3):672–681. doi: 10.7150/jca.28525

Figure 4.

Figure 4

Silencing GIHCG inhibits cervical cancer cell proliferation, induces cell apoptosis, and represses cell migration. (A) The expression of GIHCG in CaSki cells 48h after GIHCG siRNAs transfection was measured by qRT-PCR. (B) Cell viability of CaSki cells at indicated time after GIHCG siRNAs transfection was measured by Glo cell viability assays. (C) Cell proliferation of CaSki cells 48h after GIHCG siRNAs transfection was measured by EdU incorporation assays. Red color indicates EdU-positive proliferative cells. Scale bars, 100 μm. (D) Cell apoptosis of CaSki cells 48h after GIHCG siRNAs transfection was measured by TUNEL assays. Green color indicates TUNEL-positive apoptotic cells. Scale bars, 100 μm. (E) Cell migration of CaSki cells 48h after GIHCG siRNAs transfection was measured by transwell migration assays. Scale bars, 100 μm. Results are displayed as mean ± SD based on three independent biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001 by Student's t-test.