| Method |
Sitting drop |
Sitting drop |
| Plate type |
2-drop Swissci |
2-drop Swissci |
| Temperature (K) |
292 |
292 |
| Protein concentration (mg ml−1) |
5.5 |
5.8 |
| Buffer composition of protein solution |
10 mM HEPES pH 7.2, 100 mM NaCl |
10 mM HEPES pH 7.2, 100 mM NaCl |
| Composition of reservoir solution |
1.1 M ammonium sulfate, 0.1 M sodium acetate pH 6.0, 1.2%(w/v) myo-inositol |
1.0 M lithium sulfate, 0.1 M sodium acetate pH 4.0, 0.1 M barium chloride |
| Volume and ratio of drop |
200:190:10 nl protein:reservoir:seed solution |
200:190:10 nl protein:reservoir:seed solution |
| Volume of reservoir (µl) |
50 |
50 |
