| Method |
Sitting-drop vapour diffusion |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
SD-2 sitting-drop plates (IDEX) |
24-well hanging-drop plates (Hampton Research) |
24-well hanging-drop plates (Hampton Research) |
| Temperature (K) |
293 |
293 |
293 |
| Protein concentration (mg ml−1) |
5.0 |
1.3 |
5.8 |
| Buffer composition of protein solution |
PBS |
PBS |
PBS |
| Composition of reservoir solution |
10% PEG 6000, 100 mM HEPES pH 7.0 |
1.4 M KH2PO4 + 3.0 M K2HPO4 pH 7.0 |
1.4 M KH2PO4 + 3.0 M K2HPO4 pH 7.0 |
| Volume and ratio of drop |
300 nl, 1:1 |
4 µl, 1:1 |
4 µl, 1:1 |
| Volume of reservoir (µl) |
50 |
500 |
500 |