Table 3. Data collection and processing.
Values in parentheses are for the outer shell.
| N9 + TB stalk | N9 − TB stalk | N9, egg-grown virus | |
|---|---|---|---|
| Diffraction source | MX2, Australian Synchrotron | MX2, Australian Synchrotron | MX1, Australian Synchrotron |
| Wavelength (Å) | 0.95369 | 0.95369 | 1.0000 |
| Temperature (K) | 100 | 100 | 100 |
| Detector | ADSC Quantum 315r | ADSC Quantum 315r | ADSC Quantum 210r |
| Crystal-to-detector distance (mm) | 300 | 300 | 130 |
| Rotation range per image (°) | 1 | 1 | 1 |
| Total rotation range (°) | 180 | 180 | 180 |
| Exposure time per image (s) | 0.5 | 0.5 | 1 |
| Space group | P21 | I432 | I432 |
| a, b, c (Å) | 101.00, 142.33, 163.30 | 180.72, 180.72, 180.72 | 180.99, 180.99, 180.99 |
| α, β, γ (°) | 90, 91.48, 90 | 90, 90, 90 | 90, 90, 90 |
| Mosaicity (°) | 1.4 | 0.6 | 0.3 |
| Resolution range (Å) | 40.00–2.57 (2.63–2.57) | 45.20–2.30 (2.35–2.30) | 33.07–1.40 (1.44–1.40) |
| Total No. of reflections | 304776 | 2392459 | 2560840 |
| No. of unique reflections | 108611 (5654) | 22761 (1453) | 98317 (4847) |
| Completeness (%) | 77.6 (70.7) | 98.1 (92.9) | 99.0 (99.6) |
| Multiplicity | 2.7 (2.1) | 29.2 (10.5) | 26.0 (14.3) |
| 〈I/σ(I)〉 | 2.9 (1.0) | 10.35 (1.3) | 29.8 (1.8) |
| CC1/2 | 0.22 | 0.25 | 0.48 |
| R r.i.m. | 0.387 (0.885) | 0.369 (0.950) | 0.124 (0.905) |
| Overall B factor from Wilson plot (Å2) | 27.40 | 31.70 | 24.99 |