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. 2019 Jan 23;75(Pt 2):116–122. doi: 10.1107/S2053230X18018290

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type Greiner CrystalQuick 96-well plate, round bottom
Temperature (K) 277
Protein concentration (mg ml−1) 3.3
Buffer composition of protein solution 50 mM Tris–HCl pH 8.0, 0.2 M NaCl
Composition of reservoir solution 20%(w/v) PEG 6000, 100 mM Tris–HCl pH 8.0, 200 mM LiCl
Volume and ratio of drop 1.0 µl:1.0 µl
Volume of reservoir (µl) 100