Figure 5.

Simulation Results of the Mathematical Model Recapitulate the Experimental Data for Different Conditions

(A) Results of simulations showing the effect of her overexpression (her OE) on her, deltaC, mespb, and mespa expression (mRNA Amplitude).

(B) Results of simulations showing the effect of the absence of Notch (notch1a^−/− mutant), her overexpression, or Notch inhibition with DAPT treatment on synchronized expression of mespb.

(C) Results of simulations showing the effect of the absence of Notch signaling (notch1a^−/− mutant) or inhibition of Notch signaling with DAPT on mespa transcription (mRNA amplitude).

(D) Results of simulations showing the effect of mespab or mespbb overexpression on mespaa and mespb transcription. To simulate a notch1a^−/− mutant, translation rate of the DeltaC protein (psd) was set to zero. To simulate the DAPT condition, psd was set to 0 after 600 min. Overexpression of mespa and mespb genes were simulated by increasing their translation rates psm_a and psm_b from 600 to 660 min. Error bars indicate two standard errors of the mean.

(E) Results of simulations of the wild-type (WT) condition showing the pattern of mespa/b (green) and her (red) transcription. 2D PSM plots with each hexagon representing a “cell”, and line plots are shown on the top and bottom panels, respectively.

See also Videos S1, S2, S3, S4, S5, S6, and S7 and Tables S1 and S2.