(A) Structure of MTSEA (top). Sensory transduction currents recorded
from IHCs during 10 s application of 2 mM MTSEA (gray) at −80 mV holding
potential. The scale bars represent 2 s (horizontal) and 100 pA (vertical) for
each trace (A–D).
(B) IHC currents recorded at +80 mV during 10 s application of 2 mM
MTSEA (gray).
(C) Structure of negatively charged MTSES (top). Representative IHC
currents after 10 s, 10 mM MTSES application (gray) at −80 mV are
shown.
(D) IHC currents recorded at +80 mV during 10 s application of 10 mM
MTSES (gray).
(E and F) Mean (± SEM) current ratios before and after 2 mM
MTSEA (E) or 10 mM MTSES (F) application in cochlea IHC and utricle type II
cells. Substitution, membrane potential, and number of cells, from 2–10
mice/condition, are indicated at the bottom.
(G) Mean (± SEM) current ratio for utricle type II cells
expressing M412C or D569C after exposure to 10 mM MTSES alone or before 2 mM
MTSET application. MTSES was applied for 80–190 s as indicated below.