| REAGENT or RESOURCES |
SOURCE |
IDENTIFIER |
| Antibodies |
|
|
| HA Tag Antibody, mAb, Mouse |
Genscript |
Cat. No. A01244; RRID:AB_1289306 |
| Goat Anti-Mouse IgG (H+L), highly
cross-adsorbed CF770 |
Biotium |
Cat. No. 20077; RRID:AB_10559194 |
| Bacterial and Virus Strains |
|
|
| One Shot TOP10 Competent E.coli |
Thermo Fisher |
Cat. No. C404006 |
| AAV2/1 |
BCH Viral Core |
http://www.childrenshospital.org/research/centers-departmental-programs/fm-kirbyneurobiology-center/cores/viral-core |
| Chemicals, Peptides, and Recombinant
Proteins |
|
|
| Salts, acids, bases |
Sigma-Aldrich |
stock |
| DDM |
Anatrace |
Cat. No. D310 |
| FOS13 |
Anatrace |
Cat. No. F310 |
| DMEM Media |
GIBCO |
Cat. No. 11995040 |
| Sodium Butyrate |
Acros Organics |
Cat. No. 156-54-7 |
| Recombinant DNA |
|
|
| hsTMC1 |
Genscript |
Accession number NM_138691.2
|
| Software and Algorithms |
|
|
| GraphPad PRISM 6 |
GraphPad Software |
N/A |
| Relion |
Scheres,
2012 |
https://www2.mrc-lmb.cam.ac.uk/relion/index.php?title=main_page |
| The PyMOL Molecular Graphics System |
Schrodinger |
https://pymol.org/2/ |
| Clustal Omega sequence alignment software |
European Bioinformatics Institute |
https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| I-TASSER |
Zhang,
2008 |
https://zhanglab.ccmb.med.umich.edu/I-TASSER/ |
| NAMD |
Phillips et
al., 2005 |
http://www.ks.uiuc.edu/Research/namd/ |
| VMD |
Humphrey et
al., 1996 |
http://www.ks.uiuc.edu/Research/vmd/ |
| SIAS |
N/A |
http://imed.med.ucm.es/Tools/sias.html |
