Table 1.
Effects of various treatments on the clinical appearance score, breathing rate, and body weights during the observation period (results for physostigmine- and neostigmine-treated animals are combined as “treatment group”).
| Clinical score | Time (hours) | Control (mean ± SD) | Treatment (mean ± SD) | Sham (mean ± SD) |
|---|---|---|---|---|
| Breathing rate (per min) | 0 | 106 ± 7.6 (n = 18) | 108 ± 11.8 (n = 28) | 105 ± 19.3 (n = 14) |
| 15 | 107 ± 19.6 (n = 15) | 114 ± 13.7 (n = 27) | 120 ± 11.4 (n = 14) | |
| 20 | 90 ± 22.3 (n = 9) | 120 ± 17.3 (n = 16) | 115 ± 18.0 (n = 14) | |
| 24 | 90 ± 14.1 (n = 3) | 115 ± 26.6 (n = 9) | 126 ± 12.8 (n = 12) | |
|
| ||||
| Coat (0-3) | 0 | 0 ± 0 (n = 18) | 0 ± 0 (n = 28) | 0 ± 0 (n = 14) |
| 15 | 2.2 ± 0.6∗,$ (n = 15) | 2.2 ± 0.5∗,$ (n = 27) | 0.45 ± 0.3 (n = 14) | |
| 20 | 2.5 ± 0.7∗,$ (n = 9) | 2.7 ± 0.5∗,$ (n = 16) | 0.36 ± 0.7 (n = 14) | |
| 24 | 3.0 ± 0.0∗,$ (n = 3) | 2.7 ± 0.5∗,$ (n = 9) | 0.29 ± 0.5 (n = 12) | |
|
| ||||
| Movement (0-3) | 0 | 0 ± 0 (n = 18) | 0 ± 0 (n = 28) | 0 ± 0 (n = 14) |
| 15 | 1.7 ± 0.6∗,$ (n = 15) | 1.8 ± 0.6∗,$ (n = 27) | 0.25 ± 0.3 (n = 14) | |
| 20 | 2.5 ± 0.5∗,$ (n = 9) | 1.9 ± 0.8∗,$ (n = 16) | 0.1 ± 0.3 (n = 14) | |
| 24 | 3.0 ± 0.0∗,$ (n = 3) | 2.1 ± 0.7∗,$ (n = 9) | 0.14 ± 0.4 (n = 12) | |
|
| ||||
| Eyes (0-3) | 0 | 0 ± 0 (n = 18) | 0 ± 0 (n = 28) | 0 ± 0 (n = 14) |
| 15 | 2.1 ± 0.3∗,$ (n = 15) | 2.2 ± 0.6∗,$ (n = 27) | 0.4 ± 0.7 (n = 14) | |
| 20 | 2.4 ± 0.5∗,$ (n = 9) | 2.7 ± 0.5∗,$ (n = 16) | 0.2 ± 0.4 (n = 14) | |
| 24 | 2.5 ± 0.7∗,$ (n = 3) | 2.7 ± 0.5∗,$ (n = 9) | 0.3 ± 0.5 (n = 12) | |
|
| ||||
| Weight (g) | 0 | 250 ± 31.9 (n = 18) | 265 ± 40.6 (n = 28) | 248 ± 23.9 (n = 14) |
| 15 | 247 ± 43.4 (n = 15) | 256 ± 73.5 (n = 27) | 256 ± 23.3 (n = 14) | |
| 20 | 264 ± 35.6 (n = 9) | 262 ± 56.6 (n = 16) | 259 ± 22.9 (n = 14) | |
| 24 | 246 ± 44.6 (n = 3) | 282 ± 71.6 (n = 9) | 270 ± 20.9 (n = 12) | |
There was no significant difference of the breathing rates during the entire experiment in any group (p = n.s.). The body weights did not change over time in any group (p = n.s.). The clinical appearance scores for eyes, coat, and movement were significantly higher in the control and treatment group than in the sham group ($p < 0.05 vs. the sham group). At t = 15 and 24 h, the clinical appearance score was in the placebo group and treatment groups significantly higher than at t = 0 (∗p < 0.05 vs. t = 0). The data are displayed as the means ± standard deviation (SD). ∗p < 0.05 versus t = 0. $p < 0.05 versus the sham group.