Table 1.
Conditions of gel electrophoresis for ZS-509 and ZS-510 complexes with 24-mer DNA oligonucleotides (concentration of the polymers – 2 mg/mL).
Lane # | Polymer | DNA concentration, μM | DNA volume, μL | Polymer volume, μL | N:P ratio |
---|---|---|---|---|---|
1 | ZS-509 | – | 0 | 2 | – |
2 | – | 0 | 3 | – | |
3 | 10 | 2 | 3 | 290:1 | |
4 | 10 | 4 | 3 | 145:1 | |
5 | 10 | 5 | 2.5 | 100:1 | |
6 | 10 | 5 | 2 | 77:1 | |
7 | 20 | 5 | 2 | 39:1 | |
8 | 20 | 7 | 1 | 14:1 | |
9 | ZS-510 | – | 0 | 2 | – |
10 | – | 0 | 3 | – | |
11 | 10 | 2 | 3 | 290:1 | |
12 | 10 | 4 | 3 | 145:1 | |
13 | 10 | 5 | 2.5 | 100:1 | |
14 | 10 | 5 | 2 | 77:1 | |
15 | 20 | 5 | 2 | 39:1 | |
16 | 20 | 7 | 1 | 14:1 |