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. 2019 Jan 20;2019:5678548. doi: 10.1155/2019/5678548

Figure 3.

Figure 3

L-Carnitine (L-C) effect on intracellular calcium pathway. (a): Representative Western blot and relevant quantification of CaMKIIα phosphorylation in hOBs treated with 5 mM L-C. CaMKIIα activation was observed at 5 and 15 min. (b): Immunofluorescence assay and quantification of total CaMKII protein after 6 and 24h. L-C enhanced the protein content of total CaMKII compared to controls after 6h. CaMKII (red), nuclei (DAPI), and merge. The quantified signal was normalized for the total nuclei number (magnification: 40X). Data are the mean ± SD of six experiments performed with cells obtained from different donors. For immunofluorescence and Western blot analyses: Student's t test: ∗p<0.05 vs control (ctrl).