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. 2019 Feb 2;10:2041731418825378. doi: 10.1177/2041731418825378

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Decellularization of hAM: (a) thawed hAM was cut into smaller pieces while still on the carrier material. (b1–b2) To remove the epithelium mechanically, a cell scraper/ soaked cotton wool tip was gently rubbed over the surface of the hAM. (c) The hAM was fixed onto an insert with a PTFE ring with the epithelial side facing the inside of the insert. (d) The hAM was washed with either SDS/SDC or Triton/SDC on both sides. (e) The hAM was mechanically washed and rinsed on a shaker (200/min) in a closed well.