Table 1.
Semi-quantitative score of the re-cellularized hAM.
Keratinocytes | Fibroblasts | Keratinization | |
---|---|---|---|
Trypsin/cell scraper | 2-2-2-2-2 | 3-3-3-3-3 | C-C-C-C-C |
NaOH/cotton pad | 1-2-1-2-1 | 3-3-3-3-3 | B-B-A-B-B |
SDS/SDC | 1-1-1-1-1 | 3-3-3-3-3 | A-A-A-A-A |
Triton/SDC | 1-1-1-1-1 | 3-3-3-3-3 | B-A-B-A-A |
SDS: sodium dodecylsulfate; SDC: sodium deoxycholate.
Confluency: 0 = absent, 1 = focal (<50%), 2 = non-confluent (>50%), 3 = confluent layer of cells. Keratinization: A = no keratinization, B = keratinization < 50% of re-cellularized areas, C = keratinization > 50% of re-cellularized areas, D = complete keratinization.