Fig. 3.

Comparison of DNA methylation profiles of iPSC-derived HPCs with different hematopoietic cell types. a Principal component analysis demonstrates that DNAm profiles of iHPCs are distinct to other cell types. b A deconvolution algorithm of Houseman et al. [13] was used to estimate the composition of different hematopoietic cell types based on the DNAm profiles of our iHPCs (donor 1 and 2) and those of Nishizawa et al. [14]. c Heatmap of DNAm levels at CpGs that are specifically hypomethylated in specific hematopoietic cell types. iHPCs (d20) are most closely related to monocytes (CD34⁺ cells isolated from human cord blood: GSE40799; B cells, peripheral blood mononuclear cells (PBMC), CD4⁺ T cells, CD8⁺ T cells, NK cells, whole blood, granulocytes, monocytes: all GSE35069; and iPSCs: GSE37066). Gray areas indicate that no data is available for this specific CpG site. CB: cord blood