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. 2019 Feb 4;11:19. doi: 10.1186/s13148-019-0617-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Principal component analysis of DNA methylation profiles of iPSC-derived HPCs with and without co-culture. The DNA methylation profiles of iHPCs (d20; donor 1 and 2) and iPSC-derived HPCs from Nishizawa et al. [14] are closely related to those of iHPCs that were further cultured for 2 weeks with or without stromal support. Additional stromal support (iHPC on MSCs, iMSCs, and TCP, respectively) did not enhance further commitment toward the hematopoietic lineage