Fig. 3.

ZT55 induces cell apoptosis and cycle arrest at the G₂/M phase in HEL cells. (a) Apoptosis was detected by flow cytometry in HEL cells treated with ZT55 for 24, 48 and 72 h using an annexin V/PI staining method. Representative results are shown and percentage of apoptotic cells was plotted. Data are represented as mean ± SEM (n = 3). **p < 0.01, ***p < 0.001 vs. control group. (b) HEL cells were treated with ZT55 at the concentration of 12.5, 25 or 50 μM for 24 h, and cell cycle distribution was then assessed using flow cytometry. Representative results of the cell cycle analysis and histogram of cell cycle distribution. Data are represented as mean ± SEM (n = 3). ***p < 0.001 vs. control group