Table 1.
Primers for the detected Tet-R genes in selected clinical isolates and ATCC strains
| Bacteria | Genes | Primers(5′-3′) | Amplified length (bp) |
|---|---|---|---|
| L. fermentum | tetPB | F:AACTTACCCGATGGACTGGC | 146 |
| R:CCCCAATCACTTCCCCGTTT | |||
| tetT | F:CGACCCTATCCGAAGCCCTCT | 153 | |
| R:GGGCGTATCTAGGAGGGTGAGTT | |||
| otrA1 | F:GCCGTTACCGTTAGCATTAG | 195 | |
| R:TAACTTAGCCAAAAAGGAGGGGATG | |||
| otrA2 | F:ATAGCGGAGGCGTAAACTACTGGG | 166 | |
| R:TAAAGAAGGCCCTGGAACAACAC | |||
| tetW1 | F:ACGCTTTGGAGTTGGGATGT | 108 | |
| R:GTTGTTCGTGGGTCCGCTCTT | |||
| tetW2 | F:GAAGGTCAGGGCGGCGTCGTTT | 108 | |
| R:ATGCAGCCAATGGTCTACGC | |||
| tcr3 | F:GCGTTGGCTGAAGTAAAAGATGA | 177 | |
| R:CCCCACAACGAAAACCCCCACTT | |||
| L. rhamonsus | tetW | F:GCAAGACTGCGACTAACTTCATAAC | 161 |
| R:GTTCTGGACGATATGGCACTTGA | |||
| tetO | F:TGGCCTTCAATTCAAGCACATCT | 113 | |
| R:AGACTGGGGTGGCGACACTATTT | |||
| tcr3 | F: TAAGACGCCACTAAGCAGCAAAG | 131 | |
| R:CCATTGTCGGCGGGTATCTGTTA | |||
| tetT | F:ATCACATTCTTCGGGGTTACACG | 151 | |
| R:TACAGCGGTAGCAGGGGACATTG | |||
| L.plantarum | tetT | F:CATTTTGAACCGTTACGACACT | 260 |
| R:GTCGCTTCACGAAAGTCACCACC | |||
| tetO | F:CCTTTTCCACGGTCAAGACTAGC | 180 | |
| R:TCTGATTCCTGAAGATTGGGGTG | |||
| tetL | F:TAACAAGTAAGCCGTGGTCATCC | 123 | |
| R:GGATTACTTTCATTTTGCGGGGT | |||
| tcr3 | F:GTCTCATTTGTTGCCGACACTTC | 135 | |
| R:TGTGCTGCCGTTTTTTGTGGTCC | |||
| tetPB | F:ACTTGGCAAACAGCGGGGACT | 144 | |
| R:CACTGACTTCATTAGCCATA | |||
| 16 s rRNA | F:CCTACGGGAGGCAGCAGTAG | 101 | |
| R:CAACAGAGCTTTACGATCCGAAA |