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. 2019 Feb 4;26:14. doi: 10.1186/s12929-019-0507-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Knockdown of endogenous NFBD1 using lentivirus-mediated shRNA. The lentiviral expressing shNFBD1 and shControl were transfected into CNE1, CNE2 and HNE1 cells. The stable NFBD1-deficiency cells were obtained under puromycin (1 μg/ml). (a) Effects of constructed lentiviral on the expression of NFBD1 mRNA were determined by qRT-PCR. The relative expression level of NFBD1 mRNA was significantly downregulated in NFBD1-shRNA group, P < 0.01 compared with shControl group. Effects of constructed lentiviral on the expression of NFBD1 protein were determined by western blotting (b) and immunofluorescence (c). **P < 0.01