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. 2019 Feb 4;19:108. doi: 10.1186/s12879-019-3744-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of optimal amplification time and temperature. The amplification time (a) and temperature (b) were shown on the right. 0.64 pg of genomic DNA of C. neoformans var. grubii H99 (ATCC 208821) was used as templates. a amplification time. A clear test band appeared and became more solid as time extended to 10 min or more. b amplification temperature. An obvious test band could be seen over a wide range of temperature from 25 to 45 °C.