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. 2019 Feb 4;19:108. doi: 10.1186/s12879-019-3744-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Comparison of different DNA extraction methods. Genomic DNA from “spin columns method” was used as templates to repeat the above-mentioned experiments, especially evaluation of the detection limit (a) and cross-reactivity analysis (b). The detection limit is also 0.64 pg of genomic DNA each test and no cross-reactivity was observed with any other pathogens