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. 2019 Feb 4;50:8. doi: 10.1186/s13567-019-0624-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Dectin-2 is expressed in ovine ruminal mucosa tissue and OREC. A Dectin-2 expression in the spinous layer cells and basement layer cells of ovine ruminal mucosa tissue assessed by immunohistochemistry. Formalin-fixed paraffin-embedded sections of mucosa from the ovine rumen were stained with Dectin-2 Mouse mAb at 10 μg/mL. (Bottom panels) Staining with isotype-matched control antibodies served as a negative control. The picture on the right (40×) is an enlarged view of the red frame on the left side (10×). B Dectin-2 PCR results. Marker: D2000 DNA Marker; D-2: Dectin-2; N: Negative control. C Western blotting analysis of OREC cell lysates showing the Dectin-2 band (35 kDa). Blots were reacted with Dectin-2 Mouse mAb and with β-actin Mouse mAb as loading controls, and the four lanes in the figure represent four repetitions of the same lane.