Fig. 6.

CD36 mediates PA-induced activation of AKT/GSK-3β/β-catenin signaling. a Effects of knockdown and overexpression of CD36 on p-AKT, AKT, p-GSK-3β, GSK-3β and nuclear β-catenin. b IF showing effects of knockdown and overexpression of CD36 on cellular location of β-catenin in PA-treated GC cells. c and d PI3K inhibitor, LY294002 inhibited migration and invasion of MGC803. Histograms show wound-healing (%) (mag. × 40) and number of migrated and invaded cells (mag. × 200). Five random fields were selected for statistical analysis. e Expression levels of p-AKT, AKT, p-GSK-3β, GSK-3β, nuclear β-catenin in PA-treated MGC803 in the presence of LY294002, compared to controls. f IF showing effects of LY294002 on cellular location of β-catenin in PA-treated GC cells, compared to controls. Data are shown as mean ± SD of three independent experiments. *P<0.05, **P<0.01, ***P<0.001, ‘NS’ means not significant