(A) Genes within conserved syntenic blocks have
significantly lower MIS and MFS (Wilcoxon p < 2.2e-16). Syntenic genes or
“syntenic block” is defined as at least three genes in the same
order on the same chromosome as their orthologs in another species within a
25-kb search window. (B and C) Scatter plots show the
insertion site enrichment along two syntenic breakpoints (Ch13:2,110,000
−2,135,000, Chr10: 642000–666000). Each gap in synteny (white
area) is enriched for piggyBac insertions while flanked by
essential regions (green shading); black boxes represent the location of CDS.
(D and E) Circos plots indicate the syntenic
blocks of P. falciparum in relation to other Plasmodium
spp. (P. berghei, P. chabaudi, P. knowlesi, P.
vivax).