KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
| Antibodies | ||
| DM1α anti-tubulin | Sigma-Aldrich | T9026 |
| Rabbit anti-CLS-2 directed against N-term 14 amino acids of C. elegans CLS-2 (R107.6): MSRVISRSTPGGTC | Home made (purified from rabbit serum) | C2a14 |
| Bacterial and Virus Strains | ||
| OP50 E. coli | Caenorhabditis Genetics Center (CGC) | http://www.cgc.cbs.umn.edu/strain.php?id=11078 |
| HT115(DE3)E. coli | Caenorhabditis Genetics Center (CGC) | http://www.cgc.cbs.umn.edu/strain.php?id=8854 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| ATTO 565 NHS-ester | ATTO-TEC GmbH | AD 565–35 |
| Protamine sulfate | Sigma-Aldrich | P4020 |
| Clasto-Lactacystin β-Lactone (CLβL, proteasome inhibitor) | Merck Millipore | 426102 |
| 3XmCherry-EMTB | Gift from George von Dassow, University of Oregon | N/A |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Nematode Caenorhabditis elegans, strain JDU19: [5’mex-5 ::GFP::tbb-2; mCherry::his-11; cb-unc-119(+)] I; unc-119(ed3) III | Julien Dumont lab, CNRS Institut Jacques Monod, Paris | N/A |
| Nematode Caenorhabditis elegans, strain JA1559: [pJA138; 5'pie-1::mCherry::tbb-2::3'pie-1] | Julie Ahringer lab | N/A |
| Nematode Caenorhabditis elegans, strain MDX20: unc-119(ed3). ojIs1[unc-119(+) pie-1::GFP::tbb-2] | Maddox lab, University of North Carolina in Chapel Hill | N/A |
| Nematode Caenorhabditis elegans, strain JDU156 :ijmSi7 [pJD348/pSW077; 5'mex-5::GFP::tbb-2; mCherry::his-11; cb-unc-119(+)] I; cyk-1(or596ts) III; unc-119(ed3) III | Julien Dumont lab | N/A |
| Nematode Caenorhabditis elegans, strain JCC49: cyk-1(or596ts) III; ltIs37[pAA64; pie-1::mCHERRY::his-58; unc-119 (+)] IV | Julie C. Canman lab | N/A |
| Nematode Caenorhabditis elegans, strain OD58: unc-119(ed3) III; ltIs38[pAA1; pie-1::GFP::PH(PLC1delta1); unc-119 (+)] | Oegema & Desai lab | N/A |
| Nematode Caenorhabditis elegans, wild-type isolate (Bristol) | Caenorhabditis Genetics Center (CGC) | N2 |
| Sea urchin Paracentrotus lividus | Station Biologiques de Roscoff et Banyuls-sur-mer, France http://www.embrc-france.fr/fr | N/A |
| Oligonucleotides | ||
| Forward primer for production of dsRNA
targeting cls-2 R107.6:
TAATACGACTCACTATAGGttcaaggaaaagttggacc (Uppercase: T3 sequence) |
(Maton et al., 2015) | N/A |
| Reverse primer for production of dsRNA
targeting cls-2 R107.6:
AATTAACCCTCACTAAAGGggtgcatttctgattccacc (Uppercase: T7 sequence) |
(Maton et al., 2015) | N/A |
| Forward primer for production of dsRNA
targeting ptr-2 C32E8.8:
TAATACGACTCACTATAGGtgtgcccgatcattctgcat (Uppercase: T3 sequence) |
This study | N/A |
| Reverse primer for production of dsRNA
targeting ptr-2 C32E8.8:
AATTAACCCTCACTAAAGGgtggccatccaagagctgat (Uppercase: T7 sequence) |
This study | N/A |
| Forward primer for production of dsRNA against C27D9.1 using L4440 feeding vector in HT115 bacteria: tcagcaaccagcacattctc | https://www.sourcebioscience.com/products/life-science-research/clones/rnai-resources/c-elegans-rnai-collection-ahringer/ | SourceBioscience Location II-4A13 |
| Reverse primer for production of dsRNA against C27D9.1 using L4440 feeding vector in HT115 bacteria: aaaatacgcttgacgttggc | https://www.sourcebioscience.com/products/life-science-research/clones/rnai-resources/c-elegans-rnai-collection-ahringer/ | SourceBioscience Location II-4A13 |
| Forward primer for production of dsRNA against cls-2 (R107.6) using L4440 feeding vector in HT115 bacteria: aaatcgtcctcgaacattgg | https://www.sourcebioscience.com/products/life-science-research/clones/rnai-resources/c-elegans-rnai-collection-ahringer/ | SourceBioscience Location III-4J10 |
| Reverse primer for production of dsRNA against cls-2 (R107.6) using L4440 feeding vector in HT115 bacteria: tgatgggttttgacaaacga | https://www.sourcebioscience.com/products/life-science-research/clones/rnai-resources/c-elegans-rnai-collection-ahringer/ | SourceBioscience Location III-4J10 |
| Recombinant DNA | ||
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/index.html |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html |
| GraphPad PRISM 6.0 | GraphPad | https://www.graphpad.com/ |
| Imaris | Bitplane | http://www.bitplane.com/imaris/imaris |
| Cytosim | Nédélec Laboratory | http://github.com/nedelec/cytosim |
| Other | ||