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. 2019 Feb 1;7:e6357. doi: 10.7717/peerj.6357

Table 4. List of statistics information of five DNA barcodes and the result of incongruence length difference (ILD) analysis between ITS and each chloroplast locus.

DNA region ITS trnH-psbA matK rbcL trnK
PCR success (%) 92.7 77 89.6 91.6 72.9
Sequencing success (%) 100 96.18 95.42 95.42 95.42
Aligned sequence length (bp) 656 444 711 634 656
No. indel (length in bp) 3 (1) 5 (1–3) 0 0 4 (1)
No. variated sites 111 22 18 8 28
No. sampled species (individual) 19 (131) 19 (131) 19 (131) 19 (131) 19 (131)
Interspecific distance mean (range) (%) 0.011 (0-0.028) 0.004(0–0.028) 0.003(0–0.008) 0.002(0–0.006) 0.004(0–0.012)
Intraspecific distance mean (range) (%) 0.001(0–0.005) 0.002(0–0.021) 0.001(0–0.006) 0.001(0–0.006) 0.001(0–0.009)
p values of ILD test between ITS 0.02 0.001 0.12 0.001