Table 4. List of statistics information of five DNA barcodes and the result of incongruence length difference (ILD) analysis between ITS and each chloroplast locus.
DNA region | ITS | trnH-psbA | matK | rbcL | trnK |
---|---|---|---|---|---|
PCR success (%) | 92.7 | 77 | 89.6 | 91.6 | 72.9 |
Sequencing success (%) | 100 | 96.18 | 95.42 | 95.42 | 95.42 |
Aligned sequence length (bp) | 656 | 444 | 711 | 634 | 656 |
No. indel (length in bp) | 3 (1) | 5 (1–3) | 0 | 0 | 4 (1) |
No. variated sites | 111 | 22 | 18 | 8 | 28 |
No. sampled species (individual) | 19 (131) | 19 (131) | 19 (131) | 19 (131) | 19 (131) |
Interspecific distance mean (range) (%) | 0.011 (0-0.028) | 0.004(0–0.028) | 0.003(0–0.008) | 0.002(0–0.006) | 0.004(0–0.012) |
Intraspecific distance mean (range) (%) | 0.001(0–0.005) | 0.002(0–0.021) | 0.001(0–0.006) | 0.001(0–0.006) | 0.001(0–0.009) |
p values of ILD test between ITS | – | 0.02 | 0.001 | 0.12 | 0.001 |