Table 7. Species resolution using the Best Close Match method and the tree-based method with five barcodes and their combinations.

Sequences	Number	Best close match (%)					BI (%)	NJ (%)
		Correct	Ambiguous	Incorrect	No match	Threshold
ITS	132	93.2	6.8	0.0	0.0	0.45	84.2	84.2
trnK	125	36.0	61.6	2.4	0.0	0.91	15.8	15.8
matK	125	16.8	83.2	0.0	0.0	0.56	10.5	10.5
psbA	126	27.0	71.4	0.8	0.8	1.12	5.2	5.2
rbcL	125	12.0	88.0	0.0	0.0	0.63	0.0	0.0
ITS+trnK	125	98.4	0.0	1.6	0.0	0.53	79.0	84.2
ITS+matk	125	96.0	3.2	0.8	0.0	0.36	79.0	84.2
ITS+psbA	126	96.0	4.0	0.0	0.0	0.54	84.2	89.5
ITS+rbcL	125	100.0	0.0	0.0	0.0	0.38	89.5	89.5
trnK+matK	125	52.0	45.6	2.4	0.0	0.72	26.3	26.3
trnK+psbA	125	52.0	44.8	3.2	0.0	0.99	21.1	21.1
trnK+rbcL	125	37.6	60.8	1.6	0.0	0.77	15.8	15.8
matK+psbA	125	49.6	48.8	1.6	0.0	0.77	21.1	15.8
matK+rbcL	125	25.6	74.4	0.0	0.0	0.59	10.5	10.5
psbA+rbcL	125	30.4	68.8	0.8	0.0	0.83	10.5	5.2
ITS+matK+psbA	125	96.0	3.2	0.8	0.0	0.54	68.4	89.5
ITS+trnK+matK	125	98.4	0.0	1.6	0.0	0.54	73.7	89.5
ITS+trnK+rbcL	125	98.4	0.0	1.6	0.0	0.51	84.2	89.5
ITS+matK+rbcL	125	99.2	0.0	0.8	0.0	0.39	79.0	89.5
ITS+rbcL+psbA	125	100.0	0.0	0.0	0.0	0.57	79.0	94.7
ITS+trnK+psbA	125	98.4	0.0	1.6	0.0	0.68	79.0	89.5
trnK+matK+rbcL	125	52.0	45.6	2.4	0.0	0.69	26.3	26.3
trnK+matK+psbA	125	63.2	35.2	1.6	0.0	0.82	26.3	26.3
matK+psbA+rbcL	125	49.6	49.6	0.8	0.0	0.72	21.1	21.1
rbcL+trnK+psbA	125	55.2	41.6	3.2	0.0	0.86	15.8	21.1
ITS+matK+psbA+rbcL	125	99.2	0.0	0.8	0.0	0.57	68.4	84.2
ITS+matK+psbA+trnK	125	98.4	0.0	1.6	0.0	0.64	73.7	84.2
ITS+matK+rbcL+trnK	125	98.4	0.0	1.6	0.0	0.52	73.7	84.2
ITS+rbcL+trnK+psbA	125	98.4	0.0	1.6	0.0	0.66	79.0	84.2
trnK+matK+psbA+rbcL	125	63.2	35.2	1.6	0.0	0.77	26.3	26.3
ITS+trnK+matK+psbA+rbcL	125	98.4	0.0	1.6	0.0	0.64	79.0	84.2