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. Author manuscript; available in PMC: 2019 Feb 4.
Published in final edited form as: Mol Pharm. 2018 Oct 10;15(11):4947–4962. doi: 10.1021/acs.molpharmaceut.8b00592

Table 1.

Analytical Assays To Characterize Carrier Proteins and Oxycodone Conjugate Vaccinesa

Test compound MALDI-TOF DLS SEC-HPLC SDS PAGE MW (kDa) HR
KLH X √ X N/A
OXY-KLH X √ X N/A
rTTHc √ √ X √ 52.5
OXY-rTTHc √ √ X X 57.3 15
EcoCRM √ √ X √ 59.0
OXY-EcoCRM √ √ X X 66.9 24
TT X √ N/A N/A
OXY-TT X √ N/A N/A
CRM197 √ √ N/A N/A 58.8
OXY-CRM197 √ X N/A N/A 68.0 28
a

When possible, unconjugated and conjugated proteins were characterized with MALDI-TOF, DLS, SEC-HPLC, and SDS PAGE. The table reports the haptenation ratio (HR) according to the molecular weight (MW) as measured by MALDI-TOF.