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. 2018 Dec 21;8(1):bio039511. doi: 10.1242/bio.039511

Fig. 5.

Fig. 5.

Changes in phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] content after membrane cholesterol depletion by MβC in RTX neuropathy. PI(4,5)P2 content was determined through the enzyme-linked immunosorbent assay following a nonlinear fit regression of the PI(4,5)P2 standard concentration as described in the Materials and Methods. (A) Nonlinear fit regression revealed that the PI(4,5)P2 content was fitted to the optical densities of the standard curve (P=0.9934) determined using a 450 nm wavelength. (B) Changes in PI(4,5)P2 content in the vehicle (open circle, n=6), MβC alone (filled circle, n=5), Sal+RTX (open square, n=5), MβC pre-RTX (open diamond, n=5), and MβC post-RTX groups (open hexagon, n=8). For the MβC pre-RTX group, MβC was delivered at RTXd0, RTXd1, RTXd3 and RTXd5. For the Sal+RTX group, animals with RTX neuropathy received additional saline as control. For the MβC alone group, naïve mice received MβC as per the schedule of the MβC pre-RTX group. For the MβC post-RTX group, MβC was delivered at RTXd7, RTXd9, RTXd11 and RTXd13. *P<0.05, **P<0.01: Sal+RTX, MβC pre-RTX and MβC post-RTX groups compared with the MβC alone group, respectively. ##P<0.01, ###P<0.001: Sal+RTX and MβC post-RTX groups compared with the MβC pre-RTX group, respectively. ξP<0.05, ξξP<0.01: MβC alone and MβC pre-RTX groups compared with the vehicle group, respectively.