Fig. 3.

Cell migration was enhanced by culture media supernatant from IL-31-stimulated alveolar epithelial cells. Alveolar epithelial cells were treated with 100 ng/ml recombinant IL-31 for 24 h. Culture media supernatant was added to the lower chamber of Transwell plates and cell suspensions of macrophages or T lymphocytes was added to the upper chamber. Migrated cells were counted under a fluorescence microscope at 3 h and 6 h, respectively. Culture media from IL-31-stimulated cells induced greater cell migration than the controls. (A) Macrophages (n=5). (B) T lymphocytes (n=5). Shown is the mean (±s.d.) of five replicates per condition at each time point for one experiment. Significance was determined by two-tailed Student's t-tests for two groups. ***P<0.001. The experiment was replicated twice.