Figure 4.

Measurement of NO level in hepatocytes in response to REM sleep loss and sleep recovery. Rats were REM sleep deprived of 4 days and 9 days before recovery of 5 days. The hepatocytes were collected from different time points and cultured for nitrite measurement. The X-axis represents the days of REM sleep deprivation and recovery and Y-axis represents the nitrite concentration (µM) in response to REM sleep deprivation. The Kit (Griess reagent kit, Sigma) was used to measure out the levels of nitrite from cultured hepatocytes. [M=Media only, CC=cage control, LPC=large platform control, REM=rapid eye movement), refer figure 2 for statistical details and other legend abbreviation]