Table 3.

In-house NAAT tests for NiV*

Assay type	Target	Reference laboratory	Reference
RT-PCR	NiV P, N genes	ICDDRB and IEDCR, CDC (USA)	7 45
Real-time RT-PCR	NiV N gene	Institut Pasteur (France)	112
SYBR qRT-PCR	NiV N gene	University of Malaya (Malaysia)	113
Duplex nested RT-PCR	NiV N gene	Chulalongkorn University Hospital (Thailand)	114
Nested RT-PCR	HeV/NiV P gene	Bernhard Nocht Institute (Germany), CDC (USA)	18 115
Nested RT-PCR	NiV L gene	CSIRO/AAHL, University of Cambridge, Zoological Society (UK)	71
SYBR qRT-PCR	NiV N gene	CSIRO/AAHL, University of Cambridge, Zoological Society (UK)	71
Real-time RT-PCR	NiV/HeV P, M, N genes	CSIRO/AAHL, University of Cambridge, Zoological Society (UK)	71
Multiplex bead-based real-time RT-PCR	NiV, HeV N and P genes	CSIRO/AAHL (Australia)	89
Multiplex array card real-time RT-PCR	26 multiplex panel including NiV N gene	CDC (Kenya, USA)	90
Multiplex array card real-time RT-PCR	21 multiplex panel including NiV N gene	Kenya Medical Research Institute (Kenya), CDC (Kenya, USA)	91

*Adapted with permission from Springer Customer Service Centre GmBH: Springer Nature Indian Journal of Virology,⁶⁶ copyright 2013; Springer Customer Service Centre GmBH: Springer Nature Springer eBook,⁶⁷ copyright 2012; and World Organisation for Animal Health (OIE).⁷⁰

AAHL, Australian Animal Health Laboratory; CDC, Centers for Disease Control and Prevention; CSIRO, Commonwealth Scientific and Industrial Research Organisation; HeV, Hendra virus; ICDDRB, International Centre for Diarrhoeal Disease Research, Bangladesh; IEDCR, Institute of Epidemiology, Disease Control and Research; NiV, Nipah virus; RT-PCR, reverse transcriptase PCR; qRT-PCR, quantitative RT-PCR.