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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Mol Reprod Dev. 2018 Jul;85(7):635–648. doi: 10.1002/mrd.23001

Figure 1.

Figure 1.

Detection of SMCHD1. A. Western blot testing of SMCHD1 antibody specificity. Blot was reacted withantibodies to mouse SMCHD1 and Actin. M, protein ladder; 1, embryo stem cells; 2, mouse embryonic fibroblasts. B. Expression of SMCHD1 in preimplantation embryos. Immunofluorescence confocal microscopy analysis of SMCHD1 expression during preimplantation development. Results were replicated across 2 independent collections for each stage. Embryos were fixed at the following times (hours post-hCG injection) during in vitro culture: 1C (n=16, 24 h), E2C (n=6, 32 h), M2C (n=8, 41 h), L2C (n=7, 44 h), E4C (n=16, 51 h), M4C (n=12, 57 h), L4C (n=21, 67 h), M8C (n=10, 76 h), LMor (n=6, 92 h), EB (n=10, 92 h), XB (n=7, 116 h), , and LB (n=6, 120 h). Note the periodic enrichment of SMCHD1 in nuclei at mid 2-cell (M2C), 4-cell (4C), 8-cell (8C) and morula (Mor) stages. E = early, M = mid, L = late. Mor = morula, EB = early blastocyst, XB = expanded blastocyst, HB = hatched blastocyst. Bar = 20 μm.