Table 2.
Primers for qPCR
| Target | Assay | Sequence/Catalog # | Primer Efficiency (%) |
|---|---|---|---|
| Arid3a | Taqman | Mm00492248_m1 | 96.6 |
| β-Actin | Syber Green | F-CTGAACCCTAAGGCCAACC R-CCAGAGGCATACAGGGACAG |
|
| Cdx2 | Syber Green | F-AGACAAATACCGGGTGGTGTA R-CCAGCTCACTTTTCCTCCTGA |
|
| Dppa1 | Taqman | Mm00626454_m1 | 92.9 |
| Eomes | Syber Green | F-TGTGACGGCCTACCAAAACA R-GCCGTGTACATGGAATCGTAG |
|
| Igf2r | Taqman | Mm00439576_m1 | 96.5 |
| Krt8 | Syber Green | F-CTTCATTGACAAGGTGCGCTT R-TTGCTCCTCGACGTCTTCTG |
|
| Nt5c3b | Taqman | Mm00518281_m1 | 92.4 |
| Peg10 | Taqman | Mm01167724_m1 | 100 |
| Rpl18 | Taqman | Mm02745785_g1 | 100 |
| Skp2 | Taqman | Mm00449925_m1 | 97.6 |
| Smchd1 | Taqman | Mm00512253_m1 | 89.3 |
| Smchd1 | Syber Green | F-TACTGACGGCGACGAAAGAAA R-CTCTGCAAGAGCAAATGGCAAA |
|
| Ubtf | Taqman | Mm00456972_m1 | 93.9 |