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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Mol Reprod Dev. 2018 Jul;85(7):635–648. doi: 10.1002/mrd.23001

Table 8.

Quantitative RT-PCR analysis of mRNA expression changes with Smchd1 siRNA knockdown at morula and blastocyst stages.

Morula Blastocyst
Gene FC1 p-value2 q-value3 n4 FC1 p-value2 q-value3 n4
Arid3a** −1.034 0.867 0.867 5 N/A N/A N/A
Dppa1* 3.619 0.002 0.014 6 N/A N/A N/A
Igf2r* −1.383 0.028 0.039 6 N/A N/A N/A
Nt5c3b* −3.480 0.011 0.039 5 N/A N/A N/A
Nt5c3b** −4.247 0.00007 0.001 7 N/A N/A N/A
Peg10** −1.055 0.431 0.464 4 1.461 0.023 0.036 3
Skp2* −1.619 0.018 0.032 5 N/A N/A N/A
Skp2** −1.441 0.002 0.009 7 N/A N/A N/A
Smchd1* −6.441 0.014 0.033 5 N/A N/A N/A
Smchd1** −4.842 0.012 0.034 5 −1.830 0.017 3
Tfap2a* 1.402 0.416 0.529 5 1.074 0.428 3
1)

Fold-change (positive: upregulated by Smchd1 siRNA knockdown, negative: downregulated); relative expression values (RQs) were calculated using Rpl18 as the internal reference gene.

2)

T-test p-value.

3)

q = p-value adjusted by Benjamini-Hochberg procedure

4)

Number of biological replicates per group.

*

SPIA amplified cDNA as template

**

Unamplified cDNA as template, N/A, not analyzed.